应(Ying)用(Yong)案(An)例(Li)
应(Ying)用(Yong)案(An)例(Li)
点(Dian)击(Ji)蓝(Lan)字(Zi)
关(Guan)注(Zhu)我(Wo)们(Men)FOLLOW US
研(Yan)究(Jiu)背(Bei)景(Jing)
甲(Jia)烷(Wan)((?)CH4)(?)是(Shi)仅(Jin)次(Ci)于(Yu)二(?)氧(Yang)化(Hua)碳(Tan)((?)CO2)(?)的(De)第(Di)二(?)大(Da)人(Ren)类(Lei)排(Pai)放(Fang)的(De)温(Wen)室(Shi)气(Qi)体(Ti)。(?)自(Zi)工(Gong)业(Ye)时(Shi)代(Dai)以(Yi)来(Lai),(?)大(Da)气(Qi)中(Zhong)的(De)甲(Jia)烷(Wan)浓(Nong)度(Du)从(Cong)工(Gong)业(Ye)化(Hua)前(Qian)的(De)715ppbv增(Zeng)加(Jia)到(Dao)目(Mu)前(Qian)的(De)约(Yue)2000ppbv。(?)虽(Sui)然(Ran)甲(Jia)烷(Wan)的(De)浓(Nong)度(Du)远(Yuan)低(Di)于(Yu)二(?)氧(Yang)化(Hua)碳(Tan),(?)但(Dan)其(Qi)全(Quan)球(Qiu)变(Bian)暖(Nuan)潜(Qian)力(Li)((?)GWP)(?)约(Yue)为(Wei)二(?)氧(Yang)化(Hua)碳(Tan)的(De)30倍(Bei)到(Dao)80倍(Bei)((?)视(Shi)时(Shi)间(Jian)尺(Chi)度(Du)而(?)定(Ding))(?)。(?)因(Yin)此(Ci),(?)了(Liao)解(Jie)甲(Jia)烷(Wan)的(De)来(Lai)源(Yuan)对(Dui)于(Yu)预(Yu)测(Ce)未(Wei)来(Lai)的(De)全(Quan)球(Qiu)变(Bian)暖(Nuan)趋(Qu)势(Shi)和(He)寻(Xun)找(Zhao)减(Jian)少(Shao)温(Wen)室(Shi)气(Qi)体(Ti)排(Pai)放(Fang)的(De)解(Jie)决(Jue)方(Fang)案(An)至(Zhi)关(Guan)重(Zhong)要(Yao)。(?)
海(Hai)洋(Yang)是(Shi)一(Yi)个(Ge)重(Zhong)要(Yao)的(De)甲(Jia)烷(Wan)源(Yuan),(?)但(Dan)传(Chuan)统(Tong)上(Shang)认(Ren)为(Wei)海(Hai)洋(Yang)表(Biao)面(Mian)排(Pai)放(Fang)的(De)甲(Jia)烷(Wan)数(Shu)量(Liang)很(Hen)少(Shao),(?)这(Zhe)主(Zhu)要(Yao)是(Shi)因(Yin)为(Wei)大(Da)部(Bu)分(Fen)甲(Jia)烷(Wan)在(Zai)水(Shui)柱(Zhu)上(Shang)升(Sheng)过(Guo)程(Cheng)中(Zhong)被(Bei)微(Wei)生(Sheng)物(Wu)氧(Yang)化(Hua)掉(Diao)了(Liao)。(?)然(Ran)而(?),(?)最(Zui)近(Jin)的(De)研(Yan)究(Jiu)表(Biao)明(Ming),(?)即(Ji)使(Shi)在(Zai)氧(Yang)化(Hua)的(De)上(Shang)层(Ceng)混(Hun)合(He)层(Ceng)中(Zhong),(?)海(Hai)水(Shui)中(Zhong)也(Ye)经(Jing)常(Chang)出(Chu)现(Xian)甲(Jia)烷(Wan)过(Guo)饱(Bao)和(He)的(De)现(Xian)象(Xiang),(?)这(Zhe)暗(An)示(Shi)海(Hai)洋(Yang)甲(Jia)烷(Wan)的(De)释(Shi)放(Fang)量(Liang)可(Ke)能(Neng)被(Bei)低(Di)估(Gu)了(Liao)。(?)
赫(He)氏(Shi)圆(Yuan)石(Shi)藻(Zao)((?)Emiliania huxleyi)(?)是(Shi)一(Yi)种(Zhong)重(Zhong)要(Yao)的(De)海(Hai)洋(Yang)微(Wei)藻(Zao),(?)对(Dui)海(Hai)洋(Yang)的(De)初(Chu)级(Ji)生(Sheng)产(Chan)有(You)显(Xian)著(Zhu)贡(Gong)献(Xian),(?)并(Bing)且(Qie)已(Yi)知(Zhi)赫(He)氏(Shi)圆(Yuan)石(Shi)藻(Zao)能(Neng)够(Gou)通(Tong)过(Guo)光(Guang)合(He)作(Zuo)用(Yong)固(Gu)定(Ding)二(?)氧(Yang)化(Hua)碳(Tan)产(Chan)生(Sheng)甲(Jia)烷(Wan)。(?)然(Ran)而(?),(?)它(Ta)与(Yu)光(Guang)合(He)作(Zuo)用(Yong)活(Huo)动(Dong)相(Xiang)关(Guan)的(De)甲(Jia)烷(Wan)产(Chan)生(Sheng)量(Liang)尚(Shang)不(Bu)明(Ming)确(Que)。(?)
研(Yan)究(Jiu)目(Mu)的(De)
1. 量(Liang)化(Hua)赫(He)氏(Shi)圆(Yuan)石(Shi)藻(Zao)在(Zai)不(Bu)同(Tong)光(Guang)照(Zhao)条(Tiao)件(Jian)下(Xia)的(De)甲(Jia)烷(Wan)释(Shi)放(Fang)速(Su)率(Lu)。(?)
2. 确(Que)定(Ding)甲(Jia)烷(Wan)释(Shi)放(Fang)与(Yu)光(Guang)合(He)作(Zuo)用(Yong)和(He)碳(Tan)固(Gu)定(Ding)之(Zhi)间(Jian)的(De)关(Guan)系(Xi)。(?)
3. 评(Ping)估(Gu)赫(He)氏(Shi)圆(Yuan)石(Shi)藻(Zao)的(De)甲(Jia)烷(Wan)释(Shi)放(Fang)对(Dui)其(Qi)海(Hai)洋(Yang)生(Sheng)物(Wu)碳(Tan)泵(Beng)((?)BCP)(?)贡(Gong)献(Xian)的(De)影(Ying)响(Xiang)。(?)
研(Yan)究(Jiu)方(Fang)法(Fa)
研(Yan)究(Jiu)者(Zhe)在(Zai)人(Ren)工(Gong)海(Hai)水(Shui)中(Zhong)以(Yi)批(Pi)式(Shi)或(Huo)半(Ban)连(Lian)续(Xu)培(Pei)养(Yang)模(Mo)式(Shi)培(Pei)养(Yang)微(Wei)藻(Zao)赫(He)氏(Shi)圆(Yuan)石(Shi)藻(Zao),(?)严(Yan)格(Ge)控(Kong)制(Zhi)初(Chu)始(Shi)细(Xi)胞(Bao)密(Mi)度(Du),(?)并(Bing)使(Shi)细(Xi)胞(Bao)适(Shi)应(Ying)12小(Xiao)时(Shi)光(Guang)照(Zhao)和(He)12小(Xiao)时(Shi)黑(Hei)暗(An)的(De)周(Zhou)期(Qi),(?)以(Yi)模(Mo)拟(Ni)自(Zi)然(Ran)环(Huan)境(Jing)中(Zhong)的(De)昼(Zhou)夜(Ye)变(Bian)化(Hua)。(?)通(Tong)过(Guo)调(Diao)整(Zheng)光(Guang)照(Zhao)强(Qiang)度(Du),(?)观(Guan)察(Cha)不(Bu)同(Tong)光(Guang)照(Zhao)水(Shui)平(Ping)对(Dui)微(Wei)藻(Zao)生(Sheng)长(Chang)和(He)甲(Jia)烷(Wan)产(Chan)生(Sheng)的(De)影(Ying)响(Xiang)。(?)
仪(Yi)器(Qi)应(Ying)用(Yong)
研(Yan)究(Jiu)者(Zhe)使(Shi)用(Yong)了(Liao)Picarro G2308高(Gao)精(Jing)度(Du)气(Qi)体(Ti)分(Fen)析(Xi)仪(Yi)来(Lai)测(Ce)定(Ding)培(Pei)养(Yang)体(Ti)系(Xi)中(Zhong)甲(Jia)烷(Wan)的(De)浓(Nong)度(Du),(?)该(Gai)仪(Yi)器(Qi)能(Neng)够(Gou)检(Jian)测(Ce)低(Di)至(Zhi)1ppb的(De)浓(Nong)度(Du)变(Bian)化(Hua),(?)确(Que)保(Bao)了(Liao)痕(Hen)量(Liang)气(Qi)体(Ti)测(Ce)量(Liang)结(Jie)果(Guo)的(De)准(Zhun)确(Que)性(Xing)。(?)
同(Tong)时(Shi),(?)使(Shi)用(Yong)Multi-color PAM荧(Ying)光(Guang)仪(Yi)测(Ce)量(Liang)了(Liao)叶(Ye)绿(Lu)素(Su)a的(De)荧(Ying)光(Guang)参(Can)数(Shu),(?)包(Bao)括(Kuo)最(Zui)大(Da)量(Liang)子(Zi)产(Chan)率(Lu)(Fv/Fm)、(?)有(You)效(Xiao)量(Liang)子(Zi)产(Chan)率(Lu)(Yield)和(He)快(Kuai)速(Su)光(Guang)曲(Qu)线(Xian)(RLCs),(?)以(Yi)评(Ping)估(Gu)微(Wei)藻(Zao)的(De)光(Guang)合(He)作(Zuo)用(Yong)效(Xiao)率(Lu)和(He)电(Dian)子(Zi)传(Chuan)递(Di)速(Su)率(Lu)。(?)使(Shi)用(Yong)粒(Li)子(Zi)计(Ji)数(Shu)器(Qi)和(He)大(Da)小(Xiao)分(Fen)析(Xi)仪(Yi)监(Jian)测(Ce)了(Liao)细(Xi)胞(Bao)密(Mi)度(Du)和(He)直(Zhi)径(Jing)的(De)变(Bian)化(Hua),(?)以(Yi)得(De)到(Dao)微(Wei)藻(Zao)的(De)特(Te)定(Ding)生(Sheng)长(Chang)率(Lu)。(?)此(Ci)外(Wai),(?)通(Tong)过(Guo)添(Tian)加(Jia)光(Guang)合(He)作(Zuo)用(Yong)抑(Yi)制(Zhi)剂(Ji)DCMU,(?)探(Tan)究(Jiu)光(Guang)合(He)作(Zuo)用(Yong)过(Guo)程(Cheng)中(Zhong)电(Dian)子(Zi)传(Chuan)递(Di)对(Dui)甲(Jia)烷(Wan)产(Chan)生(Sheng)的(De)影(Ying)响(Xiang)。(?)为(Wei)了(Liao)排(Pai)除(Chu)其(Qi)他(Ta)微(Wei)生(Sheng)物(Wu)可(Ke)能(Neng)对(Dui)实(Shi)验(Yan)结(Jie)果(Guo)的(De)干(Gan)扰(Rao),(?)研(Yan)究(Jiu)者(Zhe)采(Cai)用(Yong)了(Liao)1.2 m的(De)PC膜(Mo)过(Guo)滤(Lu)培(Pei)养(Yang)后(Hou)的(De)微(Wei)藻(Zao)细(Xi)胞(Bao),(?)然(Ran)后(Hou)在(Zai)相(Xiang)同(Tong)的(De)光(Guang)照(Zhao)和(He)温(Wen)度(Du)条(Tiao)件(Jian)下(Xia)培(Pei)养(Yang)含(Han)有(You)细(Xi)菌(Jun)的(De)滤(Lu)液(Ye),(?)以(Yi)测(Ce)定(Ding)异(Yi)养(Yang)产(Chan)生(Sheng)的(De)甲(Jia)烷(Wan)。(?)同(Tong)时(Shi),(?)通(Tong)过(Guo)添(Tian)加(Jia)甲(Jia)烷(Wan)古(Gu)菌(Jun)抑(Yi)制(Zhi)剂(Ji)2-溴(Xiu)乙(Yi)磺(Huang)酸(Suan)(2-BES),(?)进(Jin)一(Yi)步(Bu)验(Yan)证(Zheng)了(Liao)微(Wei)藻(Zao)自(Zi)身(Shen)产(Chan)生(Sheng)的(De)甲(Jia)烷(Wan)。(?)
最(Zui)后(Hou),(?)研(Yan)究(Jiu)者(Zhe)通(Tong)过(Guo)一(Yi)系(Xi)列(Lie)生(Sheng)化(Hua)分(Fen)析(Xi),(?)包(Bao)括(Kuo)使(Shi)用(Yong)CHNS元(Yuan)素(Su)分(Fen)析(Xi)仪(Yi)测(Ce)定(Ding)细(Xi)胞(Bao)的(De)碳(Tan)和(He)氮(Dan)含(Han)量(Liang),(?)以(Yi)及(Ji)通(Tong)过(Guo)放(Fang)射(She)性(Xing)同(Tong)位(Wei)素(Su)标(Biao)记(Ji)技(Ji)术(Shu)测(Ce)量(Liang)光(Guang)合(He)作(Zuo)用(Yong)碳(Tan)固(Gu)定(Ding)率(Lu),(?)全(Quan)面(Mian)评(Ping)估(Gu)了(Liao)微(Wei)藻(Zao)的(De)生(Sheng)理(Li)状(Zhuang)态(Tai)和(He)代(Dai)谢(Xie)活(Huo)动(Dong)。(?)
通(Tong)过(Guo)这(Zhe)些(Xie)综(Zong)合(He)性(Xing)的(De)实(Shi)验(Yan)设(She)计(Ji)和(He)精(Jing)确(Que)的(De)测(Ce)量(Liang)技(Ji)术(Shu),(?)能(Neng)够(Gou)详(Xiang)细(Xi)地(Di)分(Fen)析(Xi)赫(He)氏(Shi)圆(Yuan)石(Shi)藻(Zao)在(Zai)不(Bu)同(Tong)光(Guang)照(Zhao)条(Tiao)件(Jian)下(Xia)的(De)甲(Jia)烷(Wan)产(Chan)生(Sheng)机(Ji)制(Zhi),(?)以(Yi)及(Ji)这(Zhe)一(Yi)过(Guo)程(Cheng)对(Dui)海(Hai)洋(Yang)生(Sheng)物(Wu)碳(Tan)泵(Beng)的(De)潜(Qian)在(Zai)影(Ying)响(Xiang),(?)为(Wei)理(Li)解(Jie)海(Hai)洋(Yang)生(Sheng)态(Tai)系(Xi)统(Tong)在(Zai)全(Quan)球(Qiu)碳(Tan)循(Xun)环(Huan)中(Zhong)的(De)作(Zuo)用(Yong)提(Ti)供(Gong)了(Liao)重(Zhong)要(Yao)的(De)科(Ke)学(Xue)依(Yi)据(Ju)。(?)
图(Tu) 1:(?)赫(He)氏(Shi)圆(Yuan)石(Shi)藻(Zao)通(Tong)过(Guo)光(Guang)合(He)作(Zuo)用(Yong)固(Gu)定(Ding)二(?)氧(Yang)化(Hua)碳(Tan)((?)CO2)(?)和(He)释(Shi)放(Fang)甲(Jia)烷(Wan)((?)CH4)(?)对(Dui)全(Quan)球(Qiu)变(Bian)暖(Nuan)产(Chan)生(Sheng)的(De)拮(Jie)抗(Kang)反(Fan)馈(Kui)效(Xiao)应(Ying),(?)光(Guang)合(He)作(Zuo)用(Yong)驱(Qu)动(Dong)的(De)甲(Jia)烷(Wan)产(Chan)量(Liang)抵(Di)消(Xiao)了(Liao)海(Hai)洋(Yang)生(Sheng)物(Wu)炭(Tan)泵(Beng)固(Gu)碳(Tan)能(Neng)力(Li)约(Yue)13%
研(Yan)究(Jiu)结(Jie)论(Lun)
实(Shi)验(Yan)结(Jie)果(Guo)表(Biao)明(Ming)海(Hai)洋(Yang)微(Wei)藻(Zao)赫(He)氏(Shi)圆(Yuan)石(Shi)藻(Zao)的(De)甲(Jia)烷(Wan)产(Chan)生(Sheng)速(Su)率(Lu)与(Yu)光(Guang)合(He)作(Zuo)用(Yong)相(Xiang)关(Guan),(?)甲(Jia)烷(Wan)的(De)产(Chan)生(Sheng)速(Su)率(Lu)随(Sui)着(Zhuo)光(Guang)照(Zhao)强(Qiang)度(Du)的(De)增(Zeng)加(Jia)而(?)显(Xian)著(Zhu)上(Shang)升(Sheng)。(?)在(Zai)实(Shi)验(Yan)中(Zhong),(?)量(Liang)化(Hua)了(Liao)赫(He)氏(Shi)圆(Yuan)石(Shi)藻(Zao)二(?)氧(Yang)化(Hua)碳(Tan)固(Gu)定(Ding)与(Yu)甲(Jia)烷(Wan)释(Shi)放(Fang)的(De)反(Fan)作(Zuo)用(Yong)影(Ying)响(Xiang),(?)甲(Jia)烷(Wan)的(De)释(Shi)放(Fang)量(Liang)相(Xiang)对(Dui)于(Yu)二(?)氧(Yang)化(Hua)碳(Tan)固(Gu)定(Ding)的(De)摩(Mo)尔(?)比(Bi)在(Zai)1.5×(?)10^-5到(Dao)6.6×(?)10^-5之(Zhi)间(Jian),(?)表(Biao)明(Ming)甲(Jia)烷(Wan)的(De)排(Pai)放(Fang)对(Dui)通(Tong)过(Guo)二(?)氧(Yang)化(Hua)碳(Tan)固(Gu)定(Ding)实(Shi)现(Xian)的(De)海(Hai)洋(Yang)生(Sheng)物(Wu)碳(Tan)泵(Beng)效(Xiao)应(Ying)有(You)显(Xian)著(Zhu)抵(Di)消(Xiao)作(Zuo)用(Yong),(?)最(Zui)高(Gao)可(Ke)达(Da)13%。(?)研(Yan)究(Jiu)还(Huan)发(Fa)现(Xian),(?)甲(Jia)烷(Wan)的(De)生(Sheng)成(Cheng)可(Ke)能(Neng)由(You)光(Guang)合(He)作(Zuo)用(Yong)过(Guo)程(Cheng)中(Zhong)产(Chan)生(Sheng)的(De)活(Huo)性(Xing)氧(Yang)((?)ROS)(?)驱(Qu)动(Dong)芬(Fen)顿(Dun)((?)Fention)(?)反(Fan)应(Ying)发(Fa)生(Sheng),(?)甲(Jia)基(Ji)化(Hua)的(De)细(Xi)胞(Bao)内(Nei)代(Dai)谢(Xie)产(Chan)物(Wu)作(Zuo)为(Wei)底(Di)物(Wu)。(?)不(Bu)同(Tong)的(De)菌(Jun)株(Zhu)对(Dui)光(Guang)照(Zhao)的(De)适(Shi)应(Ying)性(Xing)不(Bu)同(Tong),(?)这(Zhe)导(Dao)致(Zhi)了(Liao)甲(Jia)烷(Wan)产(Chan)生(Sheng)与(Yu)光(Guang)照(Zhao)强(Qiang)度(Du)之(Zhi)间(Jian)关(Guan)系(Xi)的(De)差(Cha)异(Yi)。(?)这(Zhe)些(Xie)发(Fa)现(Xian)表(Biao)明(Ming),(?)赫(He)氏(Shi)圆(Yuan)石(Shi)藻(Zao)在(Zai)全(Quan)球(Qiu)碳(Tan)循(Xun)环(Huan)和(He)气(Qi)候(Hou)变(Bian)化(Hua)中(Zhong)的(De)作(Zuo)用(Yong)比(Bi)此(Ci)前(Qian)认(Ren)为(Wei)的(De)更(Geng)加(Jia)复(Fu)杂(Za)和(He)重(Zhong)要(Yao)。(?)
更(Geng)多(Duo)实(Shi)验(Yan)数(Shu)据(Ju)请(Qing)查(Cha)看(Kan)原(Yuan)文(Wen)献(Xian)
https://doi.org/10.21203/rs.3.rs-4268781/v1
仪(Yi)器(Qi)推(Tui)荐(Jian)
原(Yuan)文(Wen)链(Lian)接(Jie)
https://www.researchsquare.com/article/rs-4268781/v16